2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Molekulýar massanyň deňeşdirilýän paýlanyşynyň kalibrleme egrisinde ulanylýan standart maddalar: insulin, mikopeptidler, glisin-glisin-tirozin-arginin, glisin-glisin-glisin
3 Enjamlar we enjamlar
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Umuman alanyňda, Sustaryň önümlerindäki aminokislotalaryň paýy Zinpronyň önümlerindäkiden ýokarydyr.
8-nji bölüm Ulanylyşyň täsirleri
Gyçky ýumurtga döwründe ýumurtga goýýan towuklaryň önümçilik görkezijilerine we ýumurtga hiline dürli mikroelement çeşmeleriniň täsiri
Önümçilik prosesi
Maksatly helasiýa tehnologiýasy
Gygyrma emulsiýa tehnologiýasy
Basyşly püskürtme we guradyş tehnologiýasy
Sowadyjy we çyglylykdan arassalamak tehnologiýasy
Öňdebaryjy daşky gurşawy gözegçilik etmek tehnologiýasy
Goşundy A: Peptidleriň deňeşdirilen molekulýar massa paýlanyşyny kesgitlemek usullary
Standartyň kabul edilmegi: GB/T 22492-2008
1 Synag Prinsipi:
Ol ýokary öndürijilikli gel filtrasiýa hromatografiýasy arkaly kesgitlenildi. Başgaça aýdylanda, gözenekli doldurgyçy stasionar faza hökmünde ulanmak bilen, bölünmek üçin nusga komponentleriniň deňeşdirilen molekulýar massa ölçegindäki tapawuda esaslanyp, 220 nm ultramelewşe siňdiriş tolkun uzynlygynyň peptid baglanyşygynda ýüze çykarylan, gel filtrasiýa hromatografiýasy arkaly deňeşdirilen molekulýar massa paýlanyşyny kesgitlemek üçin ýöriteleşdirilen maglumatlary işläp bejeriş programma üpjünçiligini (ýagny GPC programma üpjünçiligini) ulanmak bilen, hromatogrammalar we olaryň maglumatlary işlenip, soýa peptidiniň deňeşdirilen molekulýar massasynyň ölçegini we paýlanyş aralygyny almak üçin hasaplandy.
2. Reagentler
Eksperimental suw GB/T6682-de görkezilen ikinji derejeli suwuň spesifikasiýasyna laýyk gelmelidir, reagentler, ýörite düzgünlerden başga, analitik taýdan arassa bolmalydyr.
2.1 Reagentlere asetonitril (hromatografik taýdan arassa), triftorsirke turşusy (hromatografik taýdan arassa),
2.2 Molekulýar massanyň deňeşdirilýän paýlanyşynyň kalibrleme egrisinde ulanylýan standart maddalar: insulin, mikopeptidler, glisin-glisin-tirozin-arginin, glisin-glisin-glisin
3 Enjamlar we enjamlar
3.1 Ýokary öndürijilikli suwuk hromatograf (ÝÖHH): UV detektory we GPC maglumatlary işläp bejeriş programma üpjünçiligi bolan hromatograf iş stansiýasy ýa-da integrator.
3.2 Hereket edýän fazaly wakuum süzgüçleme we gazsyzlandyryş enjamy.
3.3 Elektron balans: graduslanan gymmat 0.000 1g.
4 Işleýiş ädimleri
4.1 Hromatografik şertler we ulgamyň adaptasiýa tejribeleri (salgylanma şertleri)
- 4.1.1 Hromatografik sütun: TSKgelG2000swxl300 mm×7.8 mm (içki diametri) ýa-da beloklary we peptidleri kesgitlemek üçin amatly bolan meňzeş öndürijilige eýe bolan şol bir görnüşli beýleki gel sütunlary.
- 4.1.2 Hereketli faza: Asetonitril + suw + triftorsirke kislotasy = 20 + 80 + 0.1.
- 4.1.3 Anyklaýyş tolkun uzynlygy: 220 nm.
- 4.1.4 Akym tizligi: 0,5 ml/min.
- 4.1.5 Anyklama wagty: 30 min.
- 4.1.6 Nusga inýeksiýasynyň möçberi: 20μL.
- 4.1.7 Sütün temperaturasy: otagyň temperaturasy.
- 4.1.8 Hromatografik ulgamyň anyklaýyş talaplaryna laýyk gelmegi üçin, ýokardaky hromatografik şertlerde gel hromatografik sütüniň netijeliliginiň, ýagny plastinkalaryň nazaryýet sanynyň (N) tripeptid standartynyň (Glisin-Glisin-Glisin) depeleri esasynda hasaplananda 10000-den az däldigi şertlendirildi.
- 4.2 Deňeşdiriji molekulýar massa standart egrileriniň öndürilişi
- Ýokarda görkezilen dürli deňeşdirilen molekulýar massaly peptid standart erginleri, massa konsentrasiýasy 1 mg / ml bolan, hereketli faza deňleşdirmesi arkaly taýýarlanyldy, belli bir derejede garyldy we soňra gözenek ölçegi 0,2 μm ~ 0,5 μm bolan organiki faza membranasyndan süzüldi we nusga sançyldy, soňra standartlaryň hromatogrammalary alyndy. Deňeşdirilen molekulýar massa kalibrleme egrileri we olaryň deňlemeleri deňeşdirilen molekulýar massanyň logarifmini saklamak wagtyna garşy çyzmak ýa-da çyzykly regressiýa arkaly alyndy.
4.3 Nusga bejergisi
10 ml ölçegli kolbada 10 mg nusgany takyk ölçäp, azajyk hereketli faza goşuň, 10 minutlap ultrases bilen çalkalaň, şeýdip nusga doly ereýär we garyşdyrylýar, hereketli faza bilen terazä çenli suwuklandyrylýar we soňra gözenek ölçegi 0,2μm ~ 0,5μm bolan organiki faza membranasyndan süzülýär we filtrat A.4.1-däki hromatografik şertlere laýyklykda seljerilýär.
- 5. Molekulýar massanyň deňeşdirilen paýlanyşynyň hasaplamasy
- 4.1-iň hromatografik şertlerinde 4.3-de taýýarlanan nusga erginini seljerenden soň, nusganyň deňeşdirilen molekulýar massasyny we onuň paýlanyş aralygyny nusganyň hromatografik maglumatlaryny GPC maglumatlaryny işläp düzmek programma üpjünçiligi bilen 4.2 kalibrleme egrisine goýmak arkaly alyp bolýar. Dürli peptidleriň deňeşdirilen molekulýar massalarynyň paýlanyşyny aşakdaky formula boýunça pik meýdanyny normallaşdyrmak usuly bilen hasaplap bolýar: X=A/A jemi × 100
- Formula boýunça: X - Nusgadaky umumy peptidde deňeşdirilýän molekulýar massa peptidiniň massa paýy, %;
- A - Deňeşdiriji molekulýar massaly peptidiň iň ýokary meýdany;
- Jemi A - her bir deňeşdirilýän molekulýar massa peptidiniň pik meýdanlarynyň jemi, bir onluk belgi çenli hasaplanýar.
- 6 Gaýtalanýanlyk
- Gaýtalanýan şertlerde alnan iki garaşsyz kesgitlemeleriň arasyndaky mutlak tapawut iki kesgitlemäniň ortaça arifmetiki bahasynyň 15% -inden geçmeli däldir.
- Goşundy B: Erkin aminokislotalary kesgitlemek usullary
- Standartyň kabul edilmegi: Q/320205 KAVN05-2016
- 1.2 Reagentler we materiallar
- Buzluk sirke turşusy: analitik taýdan arassa
- Perhlor turşusy: 0.0500 mol/L
- Indikator: 0.1% kristall bifşteks indikator (buzluk sirke turşusy)
- 2. Erkin aminokislotalaryň kesgitlenmesi
Nusgalar 80°C temperaturada 1 sagatlap guradyldy.
Nusgany gurak gapda goýuň, otagyň temperaturasyna çenli tebigy ýagdaýda sowadyň ýa-da ulanyp boljak temperatura çenli sowadyň.250 ml gury konus şekilli kolbanyň içine takmynan 0,1 g nusgany (0,001 g takyklykda) çekiň.Nusganyň daşky gurşawyň çyglylygyny özüne çekmeginiň öňüni almak üçin çaltlyk bilen indiki ädime geçiň25 ml buz sirkesi turşusyny goşuň we 5 minutdan köp bolmadyk wagtlap gowy garyşdyryň.2 damja kristall benewşe indikatoryny goşuňEritme gyrmyzy reňkden gutarýança, perhlor turşusynyň 0,0500 mol / L (±0,001) standart titrleme ergini bilen titrläň.
Sarp edilen standart erginiň möçberini ýazyň.
- Boş synagy şol bir wagtyň özünde geçiriň.
- 3. Hasaplama we netijeler
- Reagentdäki erkin aminokislotalaryň mukdary X massa paýy (%) hökmünde görkezilýär we şu formula boýunça hasaplanýar: X = C × (V1-V0) × 0.1445/M × 100%, şu formulada:
- C - Standart perhlor turşusy ergininiň litrde mollarda konsentrasiýasy (mol/L)
- V1 - Nusgalary standart perhlor turşusy ergini bilen titrlemek üçin ulanylan göwrüm, millilitrlerde (ml).
- Vo - Standart perhlor turşusy ergini bilen blankany titrlemek üçin ulanylan göwrüm, millilitrlerde (ml);
M - Nusganyň massasy, grammda (g).
| 0.1445: 1.00 ml standart perhlor turşusy erginine deň bolan aminokislotalaryň ortaça massasy [c (HClO4) = 1.000 mol / L]. | 4.2.3 Seriý sulfatyň standart titrleme ergini: konsentrasiýasy c [Ce (SO4) 2] = 0.1 mol/L, GB/T601 görä taýýarlanyldy. | |
| Standartlaryň kabul edilmegi: Q/70920556 71-2024 | 1. Kesgitleme prinsipi (Mysal hökmünde Fe) | Aminokislota demir kompleksleriniň suwsuz etanolda eremegi örän pes, erkin metal ionlary bolsa suwsuz etanolda ereýär, suwsuz etanolda ikisiniň arasyndaky eremegi tapawudy aminokislota demir kompleksleriniň helasiýa tizligini kesgitlemek üçin ulanyldy. |
| Formula boýunça: V1 - synag erginini titrlemek üçin sarp edilen seriý sulfat standart ergininiň möçberi, ml; | Suwsuz etanol; galan bölegi GB/T 27983-2011-däki 4.5.2-nji bend bilen birmeňzeşdir. | 3. Analiz ädimleri |
| Iki synagy parallel ýerine ýetiriň. 103±2℃-da guradylan nusganyň 0,1g-ny 1 sagatlap, 0,0001g takyklykda ölçäň, 100ml suwsuz etanol goşup, süzüň, galan bölegi 100ml suwsuz etanol bilen azyndan üç gezek ýuwup, süzüň, soňra galan bölegi 250ml konus şekilli kolba geçirip, GB/T27983-2011-iň 4.5.3-nji bendine laýyklykda 10ml kükürt kislotasynyň erginini goşuň we soňra GB/T27983-2011-iň 4.5.3-nji bendine laýyklykda "Ereýänçä gyzdyryň we soňra sowadyň" aşakdaky ädimleri ýerine ýetiriň. Şol bir wagtyň özünde boş synagy geçiriň. | 4. Demiriň umumy mukdaryny kesgitlemek | 4.1 Kesgitleme prinsipi GB/T 21996-2008-däki 4.4.1-nji bend bilen birmeňzeşdir. |
4.2. Reagentler we erginler
| 4.2.1 Garyşyk kislota: 700 ml suwa 150 ml kükürt kislotasyny we 150 ml fosfor kislotasyny goşuň we gowy garyşdyryň. | 4.2.2 Natriý difenilamin sulfonat indikator ergini: 5g/L, GB/T603 görä taýýarlanyldy. | 4.2.3 Seriý sulfatyň standart titrleme ergini: konsentrasiýasy c [Ce (SO4) 2] = 0.1 mol/L, GB/T601 görä taýýarlanyldy. | |
| 4.3 Analiz ädimleri | Iki synagy parallel ýerine ýetiriň. 0,1 g nusgany 020001 g takyklykda ölçäň, 250 ml konus şekilli kolba goýuň, 10 ml garyşyk kislota goşuň, ereýänden soň, 30 ml suw we 4 damja natriý dianilin sulfonat indikator erginini goşuň we soňra GB/T21996-2008-iň 4.4.2-nji bendine laýyklykda aşakdaky ädimleri ýerine ýetiriň. Boş synagy şol bir wagtyň özünde geçiriň. | 4.4 Netijeleriň görkezilişi | Demiriň massa paýy boýunça aminokislota demir toplumlarynyň umumy demir mukdary X1, bahasy % bilen görkezilen (1) formula boýunça hasaplanýar: |
| X1=(V-V0)×C×M×10-3×100 | V0 - boş ergini titrlemek üçin sarp edilen seriý sulfat standart ergini, ml; | V0 - boş ergini titrlemek üçin sarp edilen seriý sulfat standart ergini, ml; | C - Seriý sulfat standart ergininiň hakyky konsentrasiýasy, mol/L5. Helatlardaky demir mukdarynyň hasaplanmagyHelatdaky demiriň massa paýy boýunça X2 demriň mukdary, % bilen görkezilen gymmaty aşakdaky formula boýunça hasaplanýar: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Formula boýunça: V1 - synag erginini titrlemek üçin sarp edilen seriý sulfat standart ergininiň möçberi, ml; | V2 - boş ergini titrlemek üçin sarp edilen seriý sulfat standart ergini, ml;nom1-nusganyň massasy, g. Parallel kesgitleme netijeleriniň arifmetik ortaça bahasyny kesgitleme netijeleri hökmünde alyň we parallel kesgitleme netijeleriniň absolýut tapawudy 0,3% -den köp däl. | 0.05585 - 1.00 ml seriý sulfat standart erginine deň bolan gramlarda görkezilen demir demriň massasy C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-nusganyň massasy, g. Parallel kesgitleme netijeleriniň arifmetik ortaça bahasyny kesgitleme netijeleri hökmünde alyň we parallel kesgitleme netijeleriniň absolýut tapawudy 0,3% -den köp däl. | 6. Şelatlaşma tizliginiň hasaplanmagyŞelatasiýa tizligi X3, bahasy % bilen görkezilýär X3 = X2/X1 × 100Goşundy C: Zinpronyň helasiýa tizligini kesgitlemek usullary |
Standartyň kabul edilmegi: Q/320205 KAVNO7-2016
1. Reagentler we materiallar
a) Buzluk sirke turşusy: analitik taýdan arassa; b) Perhlor turşusy: 0.0500mol/L; c) Indikator: 0.1% kristall bifşteks indikator (buzluk sirke turşusy)
2. Erkin aminokislotalaryň kesgitlenmesi
2.1 Nusgalar 80°C-de 1 sagatlap guradyldy.
2.2 Nusgany gurak gapda ýerleşdirip, otagyň temperaturasyna çenli tebigy ýagdaýda sowatmaly ýa-da ulanyp boljak temperatura çenli sowatmaly.
2.3 Takmynan 0.1 g nusgany (0.001 g takyklykda) 250 ml gurak konus şekilli kolbanyň içine çekiň
2.4 Nusganyň daşky gurşawyň çyglylygyny özüne çekmeginiň öňüni almak üçin çaltlyk bilen indiki ädime geçiň.
2.5 25 ml buz sirkesi turşusyny goşuň we 5 minutdan köp däl wagtlap gowy garyşdyryň.
2.6 Kristal benewşe indikatorynyň 2 damjasyny goşuň.
2.7 Reňkini soňky nokady hökmünde üýtgetmezden, ergin gyrmyzy reňkden ýaşyla üýtgeýänçä, 15 sekuntlap perhlor turşusynyň 0.0500mol/L (±0.001) standart titrleme ergini bilen titrläň.
2.8 Sarp edilen standart erginiň möçberini ýazyň.
2.9 Boş synagy şol bir wagtyň özünde geçiriň.
- 3. Hasaplama we netijeler
- Katalan
- Physicochemical parameters
V1 - Nusgalary standart perhlor turşusy ergini bilen titrlemek üçin ulanylan göwrüm, millilitrlerde (ml).
Vo - Standart perhlor turşusy ergini bilen blankany titrlemek üçin ulanylan göwrüm, millilitrlerde (ml);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Salgysy: Hytaýyň Siçuan welaýatynyň Çendu şäheri, Pujiang etraby, Şouan şäherçesi, Çingpu köçesi, 147-nji jaý
Telefon: 86-18880477902
Önümler
Organiki däl mikroelementler
- Organiki mikroelementler
- Swahili dili
- Özleşdirilen hyzmat
- Tiz baglanyşyklar
Kompaniýanyň profili
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Sorag üçin basyň | © Awtorlyk hukugy - 2010-2025: Ähli hukuklar goralan. | Sahypanyň kartasy IŇ GOWY GÖZLEG Telefon |
| Tel | 86-18880477902 | Ýawa dili | E-poçta |
| 8618880477902 | Hytaýça | Fransuz dili | |
| Bird | Hytaýça | Fransuz dili | Nemes Ispança |
| Aquatic animals | Ýapon | Koreýçe | Arapça Grekçe |
| Türkçe | Italýan | ||
| Ruminant animal g/head day | January 0.75 | Indonez dili Afrikaans Şwed |
Polýakça
- Bask
- Katalan
- Physicochemical parameters
Hindi
Laos
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Şona
Bolgar
- Sebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Horwatiýa
Gollandiýaly
| Application object | Urdu Wýetnamça | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Gaiti | Hausa | Kinýarwanda Hmong Wenger |
| Piglets and fattening pigs | Igbo | Ýawa dili | Kannada Khmer kürtçe |
| Gyrgyz | Latyn | ||
| Bird | 300～400 | 45～60 | Makedon Malaý Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegiýa
- Puştu
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serb dili
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Şona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili dili
Täjik
Tamil
Telugu dili
Taý
| Application object | Urdu Wýetnamça | Content in full-value feed (mg/kg) | Efficacy |
| Ýidiş | Ýoruba | Zulu | Kinýarwanda Oriýýa Türkmen |
| Uýgur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
